The Thermal Inactivation of T4 and λ Bacteriophage

Intracellular inactivation of bacteriophage T4 early deoxyribonucleic acid.

As previously shown, a small amount of polynucleotide material is added to parental T4 deoxyribonucleic acid (DNA) molecules within the first 5 min of infection. I have asked whether this process is essential for phage replication. Two approaches-one involving decay of (32)P incorporated into this "early DNA" and the other involving photoinactivation of bromodeoxyuridine-containing early DNA-in...

Inactivation of bacteriophage T4, of poly-D-alanyl bacteriophage and of penicilloyl bacteriophage by immunospecifically isolated IgM and IgG antibodies.

Development of Human Complement System. Philip Fireman, Dolores A. Zuchowski and Paul M. Taylor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Inactivation of Bacteriophage T4, of Poly-D-alanyl Bacteriophage and of Penicylloyt Bacteriophage by Immunospecifically Isolated IgM and I...

The genome of bacteriophage T4.

Bacteriophage T4 genome.

Phage T4 has provided countless contributions to the paradigms of genetics and biochemistry. Its complete genome sequence of 168,903 bp encodes about 300 gene products. T4 biology and its genomic sequence provide the best-understood model for modern functional genomics and proteomics. Variations on gene expression, including overlapping genes, internal translation initiation, spliced genes, tra...

Thermal rescue of UV-irradiated bacteriophage T4 and biphasic mode of action of the WXY system.

When ultraviolet-irradiated bacteriophage T4 is assayed at plating temperatures ranging from 20 degrees to 40 degrees, its survival increases at the higher temperatures. This "thermal rescue" requires an intact WXY system but not the denV pyrimidine dimer excision system. Mutation rates decrease with increasing temperature, indicating that some lesions processed in a mutagenic manner at lower t...